A novel method for the normalization of microRNA RT-PCR data
نویسندگان
چکیده
منابع مشابه
Letting all have a say: A novel method for microRNA RT- PCR normalization
MicroRNAs (miRNAs) are short non-coding RNA molecules. MicroRNAs regulate mRNA transcript levels and translation. miRNA expression is measured by microarray or real-time polymerase chain reaction (RT-PCR). The findings of RT-PCR data are limited by the normalization techniques. Some commonly used endogenous controls are differentially expressed in cancer, making them inappropriate internal cont...
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The high-throughput analysis of microRNAs (miRNAs) circulating within the blood of healthy and diseased individuals is an active area of biomarker research. Whereas quantitative real-time reverse transcription polymerase chain reaction (qPCR)-based methods are widely used, it is yet unresolved how the data should be normalized. Here, we show that a combination of different algorithms results in...
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ژورنال
عنوان ژورنال: BMC Medical Genomics
سال: 2013
ISSN: 1755-8794
DOI: 10.1186/1755-8794-6-s1-s14